Translational_Unit
rbs+crtIB

Part:BBa_I742161:Design

Designed by: Chris French   Group: iGEM07_Edinburgh   (2007-10-24)


crtI (phytoene dehydrogenase) and crtB (phytoene synthase)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 93
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 500
    Illegal PstI site found at 911
    Illegal NgoMIV site found at 2061
    Illegal NgoMIV site found at 2191
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Note: the crtI coding sequence contains 2 PstI sites. In a later version of this part, these have been removed by site-directed mutagenesis but the functionality of the nutated gene has not been confirmed. This version still contains the two PstI sites. Note also that the crtB coding sequence overlaps the crtI coding sequence; for simplicity, in this version, they have not been separated.



Source

Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.

References